Daizu-net   for functional genomics research of soybean  
gene information  :    
[Last update: May 23th, 2026]

Our paper has been published. Please cite the following paper:
  - Yano et al. (2025) Nature Genetics 57, 973-985. The genomic landscape of gene-level structural variations in Japanese and global soybean Glycine max cultivars.
      DOI: 10.1038/s41588-025-02113-5


Data download 

G.max NARO standard genome reference (de novo assembly)   (28th, Jun, 2024)   
  [download]   Enrei v3.31 pseudomolecule assembly   (fasta)   *assembled by Oxford Nanoore + BioNano saphyr + Hi-C
  [download]   Enrei v3.32 annotation, original gene ID   (GFF3)   *gene prediction by Oxford Nanoore + EVM (BRAKER2, augustus, genome threader, stringtie)
  [download]   Enrei v3.32 annotation, consensus gene ID   (GFF3)   *Williams Glyma gene ID is attached if 1-to-1 paired orthologue was found.
  *GFF has been updated to v3.32. Gene ID strings of 27 genes were fixed without changing their genomic positions and exon/intron structures.
  *No changes in DNA sequences between v3.30 and v3.31. Prefix 'Enrei331_chr' is attached to the chrosomome sequence names in v3.31.


G.max NARO genome references (reference-guided)   (28th, Jun, 2024)   
  - Japanese cultivars and landraces -
  [download]   Fukuyutaka v3.12 pseudomolecule assembly   (fasta)   [1]
  [download]   Fukuyutaka v3.12 annotation, original gene ID   (GFF3)   [2]
  [download]   Himeshirazu v3.02 pseudomolecule assembly   (fasta)   [3]
  [download]   Himeshirazu v3.02 annotation, original gene ID   (GFF3)   [2]
  [download]   Kosuzu v3.01 pseudomolecule assembly   (fasta)   [3]
  [download]   Kosuzu v3.01 annotation, original gene ID   (GFF3)   [2]
  [download]   Miyagishirome v3.01 pseudomolecule assembly   (fasta)   [3]
  [download]   Miyagishirome v3.01 annotation, original gene ID   (GFF3)   [2]
  [download]   Ooyachi2 v3.03 pseudomolecule assembly   (fasta)   [3]
  [download]   Ooyachi2 v3.03 annotation, original gene ID   (GFF3)   [2]
  [download]   Tanba v3.02 pseudomolecule assembly   (fasta)   [3]
  [download]   Tanba v3.02 annotation, original gene ID   (GFF3)   [2]
  - others -
  [download]   Harosoy v3.01 pseudomolecule assembly   (fasta)   [3]
  [download]   Harosoy v3.01 annotation, original gene ID   (GFF3)   [2]
  [download]   Jack v3.01 pseudomolecule assembly   (fasta)   [3]
  [download]   Jack v3.01 annotation, original gene ID   (GFF3)   [2]
  [download]   Peking v3.01 pseudomolecule assembly   (fasta)   [3]
  [download]   Peking v3.01 annotation, original gene ID   (GFF3)   [2]
  [download]   UA4805 v3.01 pseudomolecule assembly   (fasta)   [1]
  [download]   UA4805 v3.01 annotation, original gene ID   (GFF3)   [2]
  [1] assembled by Oxford Nanoore + BioNano saphyr + ragtag
  [2] gene prediction by Oxford Nanoore + EVM (BRAKER3, augustus, genome threader, stringtie)
  [3] assembled by Oxford Nanoore + ragtag
  *These data do not include organella sequences.


Raw sequence data deposited in public database   (28th, Oct, 2023)   
  [LINK]   Nanopore, Illumina, Bionano map, and Proximo Hi-C dataset of Enrei and Fukuyutaka   (NCBI PRJNA935991)   
  [LINK]   Nanopore and Illumina dataset of seven soybean accessions (Himeshirazu, Kosuzu, Miyagishirome, Tanba, Harosoy, Jack, and Peking)   (NCBI PRJNA1029916)   
  [LINK]   Tissue-wide RNA-seq in Enrei and Williams 82 (total 44 samples)   (NCBI PRJNA934964)   


Pipeline and script      
  [LINK]   Asm2sv, a pipeline for assembly-based comparative genomics study